Lista över skärplatser för restriktionsenzym: S

Legenden om nukleobaser
Koda Nukleotid representerad
A Adenin (A)
C Cytosin (C)
G Guanin (G)
T Tymin (T)
N A, C, G eller T
M A eller C
R A eller G
W A eller T
Y C eller T
S C eller G
K G eller T
H A, C eller T
B C, G eller T
V A, C eller G
D A, G eller T

Den här artikeln innehåller en lista över de mest studerade restriktionsenzymer vars namn börjar med S. Den innehåller cirka 130 enzymer. Följande information ges:

  • Enzym : Accepterat namn på molekylen , enligt den internationellt antagna nomenklaturen och bibliografiska referenser . (Mer läsning: se avsnittet " Nomenklatur " i artikeln " Restriktionsenzym ".)
  • PDB-kod : Kod som används för att identifiera strukturen av ett protein i PDB -databasen över proteinstrukturer. 3D-atomstrukturen hos ett protein ger mycket värdefull information för att förstå de intima detaljerna i dess verkningsmekanism.
  • Källa : Organism som naturligt producerar enzymet.
  • Igenkänningssekvens : Sekvens av DNA som känns igen av enzymet och till vilken det specifikt binder.
  • Skärning : Skärställe och DNA-produkter från snittet. Igenkänningssekvensen dussintals nukleotider bort från igenkänningsstället.
  • Isoschizomerer och neoschizomerer : En isoschizomer är ett enzym som känner igen samma sekvens som ett annat. En neoschizomer är en speciell typ av isoschizomer som känner igen samma sekvens som en annan, men skär på ett annat sätt. Ett maximalt antal av 8-10 vanligaste isoschizomerer är indikerade för varje enzym men det kan finnas många fler. Neoschizomerer visas med fet och grön typsnitt (t.ex.: BamHI ). När "Ingen på datum " anges betyder det att det inte fanns några registrerade isoschizomerer i databaserna det datumet med en tydligt definierad skärplats. Isoschizomerer indikerade med vitt teckensnitt och grå bakgrund motsvarar enzymer som inte finns med i de aktuella listorna:
som i detta ej listade enzym:  EcoR70I 


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Restriktionsenzymer

S

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer
SacI Streptomyces achromogenes
5' GAGCTC 3' CTCGAG

  5' ---GAGCT C--- 3'   3' ---C TCGAG--- 5'
SacII Streptomyces achromogenes
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
Cfr42I
SacNI Streptomyces achromogenes NJH
5' GRGCYC 3' CYCGRG

  5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
SalI Streptomyces albus
5' GTCGAC 3' CAGCTG

  5' ---G TCGAC--- 3'   3' ---CAGCT G--- 5'
SalPI Streptomyces albus
5' CTGCAG 3' GACGTC

  5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'
ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI
SanDI Streptomyces sp.
5' GGGWCCC 3' CCCWGGG

  5' ---GG GWCCC--- 3'   3' ---CCCWG GG--- 5'
SapI Saccharopolyspora sp.
5' GCTCTTC 3' CGAGAAG

  5' ---GCTCTTCN NNN--- 3'   3' ---CGAGAAGNNNN --- 5'
Sari Streptomyces aureomonopodiales
5' AGGCCT 3' TCCGGA

  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, Pcel, Pme55I, Sru30DI, SseBI, SteI, StuI
SatI Staphylococcus arlettae RFL832
5' GCNGC 3' CGNCG

  5' ---GC NGC--- 3'   3' ---CGN CG--- 5'
SauI Streptomyces aureofaciens IKA 18/4
5' CCTNAGG 3' GGANTCC

  5' ---CC TNAGG--- 3'   3' ---GGANT CC--- 5'
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
Sau96I Staphylococcus aureus PS96
5' GGNCC 3' CCNGG

  5' ---G GNCC--- 3'   3' ---CCNG G--- 5'
AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI
Sau3239I Streptomyces aureofaciens
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
Sau3AI 2REU Staphylococcus aureus 3A
5' GATC 3' CTAG

  5' --- GATC--- 3'   3' ---CTAG --- 5'
Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
SauBMKI Streptomyces aureofaciens BM-K
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SauHPI Streptomyces aureofaciens R8/26
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SauLPI Streptomyces aureofaciens B-96
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SauLPII Streptomyces aureofaciens B-96
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
SauMI Staphylococcus aureus M
5' GATC 3' CTAG

  5' --- GATC--- 3'   3' ---CTAG --- 5'
BscFI, BspAI, BstKTI , ChaI , HacI, MkrAI, RalF40I, SsiAI
SauNI Streptomyces aureofaciens NMU
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SauSI Streptomyces aureofaciens 16
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SbfI Streptomyces sp. BF-61
5' CCTGCAGG 3' GGACGTCC

  5' ---CCTGCA GG--- 3'   3' ---GG ACGTCC--- 5'
Sbi68I Streptomyces bikiniensis JAM68
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
Sbo13I Shigella boydii 13
5' TCGCGA 3' AGCGCT

  5' ---TCG CGA--- 3'   3' ---AGC GCT--- 5'
SbvI Streptococcus bovis II/1
5' GGCC 3' CCGG

  5' ---GG CC--- 3'   3' ---CC GG--- 5'
ScaI Streptomyces caespitosus
5' AGTACT 3' TCATGA

  5' ---AGT ACT--- 3'   3' ---TCA TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
Dpal, Eco255I, RflFII, Zrml
SceIII Saccharomyces cerevisiae
5' GCCGGC 3' CGGCCG

  5' ---G CCGGC--- 3'   3' ---CGGCC G--- 5'
SchI Staphylococcus cohnii Lki 19-320
5' GAGTC 3' CTCAG

  5' ---GAGTCN 4 N --- 3'   3' ---CTCAGN 4 N --- 5'
SchZI Streptomyces chusanensis ZS-2
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SciI Streptoverticillium cinnamoneum
5' CTCGAG 3' GAGCTC

  5' ---CTC GAG--- 3'   3' ---GAG CTC--- 5'
BspAAI , BstVI , MlaAI , PaeR7I , SauLPII , SlaI , Sol10179I , XhoI
SciNI Spiroplasma citri ASP2
5' GCGC 3' CGCG

  5' ---G CGC--- 3'   3' ---CGC G--- 5'
BspLAI , BstHHI , CfoI , FnuDIII , Hhal , Hin6I, HinP1I, HsoI, HspAI
ScrFI Streptococcus cremoris F
5' CCNGG 3' GGNCC

  5' ---CC NGG--- 3'   3' ---GGN CC--- 5'
SdaI 2IXS Streptomyces diastaticus Ng7-324
5' CCTGCAGG 3' GGACGTCC

  5' ---CCTGCA GG--- 3'   3' ---GG ACGTCC--- 5'
SdiI Streptomyces diastaticus
5' GGCCN 5 GGCC 3' CCGGN 5 CCGG

  5' ---GGCCNNNN NGGCC--- 3'   3' ---CCGGN NNNNCCGG--- 5'
SduI Streptococcus durans RFL3
5' GDGCHC 3' CHCGDG

  5' ---GDGCH C--- 3'   3' ---C HCGDG--- 5'
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII
Secl Synechocystis sp. 6701
5' CCNNGG 3' GGNNCC

  5' ---C CNNGG--- 3'   3' ---GGNNC C--- 5'
SelI Synechococcus elongatus
5' CGCG 3' GCGC

  5' --- CGCG--- 3'   3' ---GCGC --- 5'
AccII , Bsh1236I , Bsp123I , Bsp50I , BstFNI , BstUI , BtkI , Csp68KVI
SenPT16I Salmonella enteritidis PT16
5' CGGCCG 3' GCCGGC

  5' ---C GGCCG--- 3'   3' ---GCCGG C--- 5'
AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII
SenPT14bI Salmonella enteritidis PT14b
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SepI Staphylococcus epidermidis
5' ATGCAT 3' TACGTA

  5' ---ATGCA T--- 3'   3' ---T ACGTA--- 5'
BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I
SexAI Streptomyces exfoliatus
5' ACCWGGT 3' TGGWCCA

  5' ---A CCWGGT--- 3'   3' ---TGGWCC A--- 5'
SexBI Streptomyces exfoliatus
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SexCI Streptomyces exfoliatus
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SfaI Streptococcus faecalis zymogenes
5' GGCC 3' CCGG

  5' ---GG CC--- 3'   3' ---CC GG--- 5'
SfaNI Streptococcus faecalis ND547
5' GCATC 3' CGTAG

  5' ---GCATCN 4 N NNNN--- 3'   3' ---CGTAGN 4 NNNNN --- 5'
Sfcl Streptococcus faecium
5' CTRYAG 3' GAYRTC

  5' ---C TRYAG--- 3'   3' ---GAYRT C--- 5'
SfeI Streptococcus faecalis
5' CTRYAG 3' GAYRTC

  5' ---C TRYAG--- 3'   3' ---GAYRT C--- 5'
SfiI 2F03 Streptomyces fimbriatus
5' GGCCN 5 GGCC 3' CCGGN 5 CCGG

  5' ---GGCCNNNN NGGCC--- 3'   3' ---CCGGN NNNNCCGG--- 5'
SflI Streptoverticillium flavopersicum
5' CTGCAG 3' GACGTC

  5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'
ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI
SfoI Serratia fonticola
5' GGCGCC 3' CCGCGG

  5' ---GGC GCC--- 3'   3' ---CCG CGG--- 5'
Sfr274I Streptomyces fradiae 274
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Sfr303I Streptomyces fradiae 303
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SfuI Streptomyces fulvissimus
5' TTCGAA 3' AAGCTT

  5' ---TT CGAA--- 3'   3' ---AAGC TT--- 5'
AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI
SgfI Streptomyces griseoruber
5' GCGATCGC 3' CGCTAGCG

  5' ---GCGAT CGC--- 3'   3' ---CGC TAGCG--- 5'
AsiSI,  RgaI, SfaAI 
SgrAI 3DPG 3N7B 3N78 3MGY 3MG6 Streptomyces griseus
5' CRCCGGYG 3' GYGGCCRC

  5' ---CR CCGGYG--- 3'   3' ---GYGGCC RC--- 5'
SgrBI Streptomyces griseus
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SimI Staphylococcus intermedius 6H
5' GGGTC 3' CCCAG

  5' ---GG GTC--- 3'   3' ---CCCAG --- 5'
SinI Salmonella infantis
5' GGWCC 3' CCWGG

  5' ---G GWCC--- 3'   3' ---CCWG G--- 5'
Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I , VpaK11BI
SlaI Streptomyces lavendulae
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
SleI Synechococcus leopoliensis
5' CCWGG 3' GGWCC

  5' --- CCWGG--- 3'   3' ---GGWCC --- 5'
AeuI , AglI , ApaORI , BspNI , Bst2I , EcoRII , SniI , SslI , SspAI, ZanI
Slu1777I Streptomyces lusitanus 1777
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SmåI Serratia marcescens Sb
5' CCCGGG 3' GGGCCC

  5' ---CCC GGG--- 3'   3' ---GGG CCC--- 5'
AhyI, CfrJ4I , EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI
SmiI Streptococcus milleri S
5' ATTTAAAT 3' TAAATTTA

  5' ---ATTT AAAT--- 3'   3' ---TAAA TTTA--- 5'
SmiMI Sphingobacterium mizutae M
5' CAYN 4 RTG 3' GTRN 4 YAC

  5' ---CAYNN NNRTG--- 3'   3' ---GTRNN NNYAC--- 5'
SmlI Stenotrophomonas maltophilia
5' CTYRAG 3' GARYTC

  5' ---C TYRAG--- 3'   3' ---GARYT C--- 5'
SmuI Sphingobacterium multivorum RFL21
5' CCCGC 3' GGGCG

  5' ---CCCGCNNNN NN--- 3'   3' ---GGGCGNNNNNN --- 5'
SmuEI Streptococcus mutans E
5' GGWCC 3' CCWGG

  5' ---G GWCC--- 3'   3' ---CCWG G--- 5'
Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I , VpaK11BI
SnaBI Sphaerotilus natans
5' TACGTA 3' ATGCAT

  5' ---TAC GTA--- 3'   3' ---ATG CAT--- 5'
SniI Streptomyces niveus
5' CCWGG 3' GGWCC

  5' ---CC WGG--- 3'   3' ---GGW CC--- 5'
ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI
SnoI Streptomyces novocastria
5' GTGCAC 3' CACGTG

  5' ---G TGCAC--- 3'   3' ---CACGT G--- 5'
Alw44I, ApaLI, VneI
SolI Streptoverticillium olivoverticillatum
5' GGATCC 3' CCTAGG

  5' ---G GATCC--- 3'   3' ---CCTAG G--- 5'
AccEBI, AliI, ApaCI, BamHI , Bce751I, CelI, OkrAI, SolI, SurI
Sol10179I Streptomyces olivochromogenes ST10179
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
SpaHI Synechococus parietina
5' GCATGC 3' CGTACG

  5' ---GCATG C--- 3'   3' ---C GTACG--- 5'
SpeI Sphaerotilus natans
5' ACTAGT 3' TGATCA

  5' ---A CTAGT--- 3'   3' ---TGATC A--- 5'
AhII, AclNI, Bcul
SphI Streptomyces phaeochromogenes
5' GCATGC 3' CGTACG

  5' ---GCATG C--- 3'   3' ---C GTACG--- 5'
SplI Spirulina platensis
5' CGTACG 3' GCATGC

  5' ---C GTACG--- 3'   3' ---GCATG C--- 5'
SpmI Sphingobacterium multivorum RFL21
5' ATCGAT 3' TAGCTA

  5' ---AT CGAT--- 3'   3' ---TAGC TA--- 5'
BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, Pgal, Ssp27144I
SpoI Salmonella potsdam
5' TCGCGA 3' AGCGCT

  5' ---TCG CGA--- 3'   3' ---AGC GCT--- 5'
SpuI Streptomyces pulveraceus
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
SrfI Streptomyces sp.
5' GCCCGGGC 3' CGGGCCCG

  5' ---GCCC GGGC--- 3'   3' ---CGGG CCCG--- 5'
SrlI Selenomonas ruminantium lactilytica 84
5' GCCGGC 3' CGGCCG

  5' ---G CCGGC--- 3'   3' ---CGGCC G--- 5'
Srl5DI Selenomonas ruminantium 5
5' CTGCAG 3' GACGTC

  5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'
AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI
Srl32DII Selenomonas ruminantium 32
5' GAATTC 3' CTAAG

  5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
Srl55DI Selenomonas ruminantium lactilytica 55
5' GAATTC 3' CTAAG

  5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
Srl56DI Selenomonas ruminantium 56
5' CTRYAG 3' GAYRTC

  5' ---C TRYAG--- 3'   3' ---GAYRT C--- 5'
SruI Selenomonas ruminantium 18D
5' TTTAAA 3' AAATTT

  5' ---TTT AAA--- 3'   3' ---AAA TTT--- 5'
AhaIII, DraI, PauAII
Sru4DI Selenomonas ruminantium
5' ATTAAT 3' TAATTA

  5' ---AT TAAT--- 3'   3' ---TAAT TA--- 5'
AseI, AsnI, BpoAI, PshBI, VspI
Sru30DI Selenomonas ruminantium 30
5' AGGCCT 3' TCCGGA

  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, Pcel, Pme55I, SarI, SseBI, SteI, StuI
SsbI Streptomyces skabb
5' AAGCTT 3' TTCGAA

  5' ---A AGCTT--- 3'   3' ---TTCGA A--- 5'
SscL1I Staphylococcus sp. L1
5' GANTC 3' CTNAG

  5' ---G ANTC--- 3'   3' ---CTNA G--- 5'
Sse9I Sporosarcina sp. 9
5' AATT 3' TTAA

  5' --- AATT--- 3'   3' ---TTAA --- 5'
Sse232I Streptomyces sp. RH232
5' CGCCGGCG 3' GCGGCCGC

  5' ---CG CCGGCG--- 3'   3' ---GCGGCC GC--- 5'
Sse1825I Streptomyces sp.
5' GGGWCCC 3' CCCWGGG

  5' ---GG GWCCC--- 3'   3' ---CCCWG GG--- 5'
Sse8387I Streptomyces sp. 8387
5' CCTGCAGG 3' GGACGTCC

  5' ---CCTGCA GG--- 3'   3' ---GG ACGTCC--- 5'
Sse8647I Streptomyces sp.
5' AGGWCCT 3' TCCWGGA

  5' ---AG GWCCT--- 3'   3' ---TCCWG GA--- 5'
SseAI Streptomyces sp.
5' GGCGCC 3' CCGCGG

  5' ---GG CGCC--- 3'   3' ---CCGC GG--- 5'
SseBI Streptomyces sp.
5' AGGCCT 3' TCCGGA

  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, Pcel, Pme55I, SarI, Sru30DI, SteI, StuI
SshAI Salmonella shikmonah
5' CCTNAGG 3' GGANTCC

  5' ---CC TNAGG--- 3'   3' ---GGANT CC--- 5'
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
SsiI Staphylococcus sciuri RFL1
5' CCGC 3' GGCG

  5' ---C CGC--- 3'   3' ---GGC G--- 5'
AciI
SsiAI Staphylococcus sp. A
5' GATC 3' CTAG

  5' --- GATC--- 3'   3' ---CTAG --- 5'
BscFI, BspAI, BstKTI , CpfI, HacI, MkrAI, RalF40I, SauMI
SsiBI Staphylococcus sp. B
5' GATC 3' CTAG

  5' --- GATC--- 3'   3' ---CTAG --- 5'
BscFI, BspFI, BstKTI , CpfI, HacI, MkrAI, RalF40I, SauMI
SslI Streptococcus salivarius thermophilus
5' CCWGG 3' GGWCC

  5' ---CC WGG--- 3'   3' ---GGW CC--- 5'
ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII , MvaI
SsoI Shigella sonnei 47
5' GAATTC 3' CTAAG

  5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
SsoII Shigella sonnei 47
5' CCNGG 3' GGNCC

  5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
SspI Sphaerotilus sp.
5' AATATT 3' TATAA

  5' ---AAT ATT--- 3'   3' ---TTA TAA--- 5'
Ssp1I Streptomyces sp. RFL1
5' TTCGAA 3' AAGCTT

  5' ---TT CGAA--- 3'   3' ---AAGC TT--- 5'
AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI
Ssp4800I Streptomyces sp. BMTU 4800
5' TGTACA 3' ACATGT

  5' ---T GTACA--- 3'   3' ---ACATG T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, SspBI
Ssp5230I Streptomyces sp. 5230
5' GACGTC 3' CTGCAG

  5' ---GACGT C--- 3'   3' ---C TGCAG--- 5'
AatII, ZraI
Ssp27144I Synechococcus sp.
5' ATCGAT 3' TAGCTA

  5' ---AT CGAT--- 3'   3' ---TAGC TA--- 5'
BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI
SspAI Synechococcus sp.
5' CCWGG 3' GGWCC

  5' --- CCWGG--- 3'   3' ---GGWCC --- 5'
AeuI , AorI , Bse17I , BspNI , Bst2I , EcoRII , SniI , SslI , SspAI, ZanI
SspBI Streptomyces sp.
5' TGTACA 3' ACATGT

  5' ---T GTACA--- 3'   3' ---ACATG T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, Ssp4800I
SspCI Streptomyces sp.
5' GCCGGC 3' CGGCCG

  5' ---GCC GGC--- 3'   3' ---CGG CCG--- 5'
SspD5I Staphylococcus sp. D5
5' GGTGA 3' CCACT

  5' ---GGTGAN 7 N --- 3'   3' ---CCACTN 7 N --- 5'
AsuHPI , HphI
SspD5II Staphylococcus sp. D5
5' ATGCAT 3' TACGTA

  5' ---ATGCA T--- 3'   3' ---T ACGTA--- 5'
Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I
SspRFI Synechococcus sp. RF-1
5' TTCGAA 3' AAGCTT

  5' ---TT CGAA--- 3'   3' ---AAGC TT--- 5'
AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI
SsrI Staphylococcus saprophyticus B6
5' GTTAAC 3' CAATTG

  5' ---GTT AAC--- 3'   3' ---CAA TTG--- 5'
SstI Streptomyces stanford
5' GAGCTC 3' CTCGAG

  5' ---GAGCT C--- 3'   3' ---C TCGAG--- 5'
SstII Streptomyces stanford
5' CCGCGG 3' GGCGCC

  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
Sst12I Streptomyces sp. ST-12
5' CTGCAG 3' GACGTC

  5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'
Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI
SteI Streptomyces tendae
5' AGGCCT 3' TCCGGA

  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, Pcel, Pme55I, SarI, Sru30DI, SseBI, StuI
SthI Salmonella thompson YY356
5' GGTACC 3' CCATGG

  5' ---G GTACC--- 3'   3' ---CCATG G--- 5'
Acc65I, AhaB8I, Asp718I, KpnI ,
Sth117I Streptococcus thermophilus ST117
5' CCWGG 3' GGWCC

  5' ---CC WGG--- 3'   3' ---GGW CC--- 5'
ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII , MvaI, SspAI
Sth132I Streptococcus thermophilus
5' CCCG 3' GGGC

  5' ---CCCGNNNN NNNN--- 3'   3' ---GGGCNNNNNNNN --- 5'
Sth134I Streptococcus thermophilus 134
5' CCGG 3' GGCC

  5' ---C CGG--- 3'   3' ---GGC C--- 5'
Sth368I Streptococcus thermophilus CNRZ368
5' GATC 3' CTAG

  5' --- GATC--- 3'   3' ---CTAG --- 5'
BscFI, BspFI, BstKTI , CpfI, HacI, MkrAI, RalF40I, SauMI
StrI Streptomyces thermodiastaticus
5' CTCGAG 3' GAGCTC

  5' ---C TCGAG--- 3'   3' ---GAGCT C--- 5'
AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
StsI Streptococcus sanguis 54
5' GGATG 3' CCTAC

  5' ---GGATGN 8 NN NNNN--- 3'   3' ---CCTACN 8 NNNNNN --- 5'
StuI Streptomyces tubercidicus
5' AGGCCT 3' TCCGGA

  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, Pcel, SarI, Sru30DI, SseBI, SteI
StyI Salmonella typhi
5' CCWWGG 3' GGWWCC

  5' ---C CWWGG--- 3'   3' ---GGWWC C--- 5'
StyD4I Salmonella typhi D4
5' CCNGG 3' GGNCC

  5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
SuaI Sulfolobus acidocaldarius
5' GGCC 3' CCGG

  5' ---GG CC--- 3'   3' ---CC GG--- 5'
SuiI Sulfolobus islandicus REN2HI
5' GCWGC 3' CGWCG

  5' ---G CWGC--- 3'   3' --- CGWC G--- 5'
AceI, ApeKI, Taq52I, Tsel
SunI Synechococcus uniformis
5' CGTACG 3' GCATGC

  5' ---C GTACG--- 3'   3' ---GCATG C--- 5'
SurI Sporosarcina ureae 2
5' GGATCC 3' CCTAGG

  5' ---G GATCC--- 3'   3' ---CCTAG G--- 5'
AccEBI, AliI, ApaCI, AsiI, BamHI , Bce751I, OkrAI, RspLKII, SolI
SviI Streptomyces violochromogenes
5' TTCGAA 3' AAGCTT

  5' ---TT CGAA--- 3'   3' ---AAGC TT--- 5'
AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I
SwaI Staphylococcus warneri
5' ATTTAAAT 3' TAAATTTA

  5' ---ATTT AAAT--- 3'   3' ---TAAA TTTA--- 5'

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