Lista över skärplatser för restriktionsenzym: G–K

Huvudartiklar: Lista över skärplatser för restriktionsenzym och restriktionsenzym
Legenden om nukleobaser
Koda Nukleotid representerad
A Adenin (A)
C Cytosin (C)
G Guanin (G)
T Tymin (T)
N A, C, G eller T
M A eller C
R A eller G
W A eller T
Y C eller T
S C eller G
K G eller T
H A, C eller T
B C, G eller T
V A, C eller G
D A, G eller T

Den här artikeln innehåller en lista över de mest studerade restriktionsenzymer vars namn börjar med G till K. Den innehåller cirka 90 enzymer.

Följande information ges:

  • Enzym : Accepterat namn på molekylen , enligt den internationellt antagna nomenklaturen och bibliografiska referenser . (Mer läsning: se avsnittet " Nomenklatur " i artikeln " Restriktionsenzym ".)
  • PDB-kod : Kod som används för att identifiera strukturen av ett protein i PDB -databasen över proteinstrukturer. 3D-atomstrukturen hos ett protein ger mycket värdefull information för att förstå de intima detaljerna i dess verkningsmekanism.
  • Källa : Organism som naturligt producerar enzymet.
  • Igenkänningssekvens : Sekvens av DNA som känns igen av enzymet och till vilken det specifikt binder.
  • Skärning : Skärställe och DNA-produkter från snittet. Igenkänningssekvensen dussintals nukleotider bort från igenkänningsstället.
  • Isoschizomerer och neoschizomerer : En isoschizomer är ett enzym som känner igen samma sekvens som ett annat. En neoschizomer är en speciell typ av isoschizomer som känner igen samma sekvens som en annan, men skär på ett annat sätt. Ett maximalt antal av 8-10 vanligaste isoschizomerer är indikerade för varje enzym men det kan finnas många fler. Neoschizomerer visas med fet och grön typsnitt (t.ex.: BamHI ). När "Ingen på datum " anges betyder det att det inte fanns några registrerade isoschizomerer i databaserna det datumet med en tydligt definierad skärplats. Isoschizomerer indikerade med vitt teckensnitt och grå bakgrund motsvarar enzymer som inte finns med i de aktuella listorna:
som i detta ej listade enzym:  EcoR70I 


Hela listans navigering

Restriktionsenzymer

G

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer
GalI Gluconobacter albidus
5' CCGCGG 3' GGCGCC
  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
GceI Gluconobacter cerinus
5' CCGCGG 3' GGCGCC
  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
GceGLI Gluconobacter cerinus
5' CCGCGG 3' GGCGCC
  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
GdiI Gluconobacter dioxyacetonicus
5' AGGCCT 3' TCCGGA
  5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5' 		AatI, AspMI, Eco147I, PeI, SarI, Sru30DI, SseBI, SteI, StuI
GdiII Gluconobacter dioxyacetonicus
5' CGGCCR 3' GCCGGY
  5' ---C GGCCR--- 3'   3' ---GCCGG Y--- 5'
GstI Geobacillus stearothermophilus
5' GGATCC 3' CCTAGG
  5' ---G GATCC--- 3'   3' ---CCTAG G--- 5'
GsuI Gluconobacter suboxydans H-15T
5' CTGGAG 3' GACCTC
  5' ---CTGGAGN 12 NNNN --- 3'   3' ---GACCTCN 12 NN NN--- 5'

H

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer
Hacl Halococcus acetoinfaciens
5' GATC 3' CTAG
  5' --- GATC--- 3'   3' ---CTAG --- 5' 		Bme12I, Bsp67I, BstENII, CcyI, FnuEI, MgoI, NphI, Sau3AI
HaeI Haemophilus aegyptius
5' WGGCCW 3' WCCGGW
  5' ---WGG CCW--- 3'   3' ---WCC GGW--- 5'
HaeII Haemophilus aegypticus
5' RGCGCY 3' YCGCGR
  5' ---RGCGC Y--- 3'   3' ---Y CGCGR--- 5'
AccB2I,  BfoI,  Bme142I ,
Bsp143II, BstH2I, Lpnl
HaeIII Haemophilus aegypticus
5' GGCC 3' CCGG
  5' ---GG CC--- 3'   3' ---CC GG--- 5' 		BsuRI
HaeIV Haemophilus aegyptius
5' GAYN 5 RTC 3' CTRN 5 YAG
  5' ---GAYN 5 RTCN 8 NNNNNN --- 3'   3' ---CTRN 5 YAGN 8 N NNNNN--- 5'
HalI Hafnia alvei B6
5' GAATTC 3' CTAAG
  5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
HallII Hafnia alvei B6
5' CTGCAG 3' GACGTC
  5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5' 		AjoI, AliAJI, Bsp63I, CfrA4I, CfuII, CstI, PstI, SflI, Srl5DI, Sst12I
HapII Haemophilus aphrophilus
5' CCGG 3' GGCC
  5' ---C CGG--- 3'   3' ---GGC C--- 5'
Hgal Haemophilus gallinarum
5' GACGC 3' CTGCG
  5' ---GACGCN 4 N NNNNN--- 3'   3' ---CTGCGN 4 NNNNNN --- 5'
HgiI Herpetosiphon giganteus 3303
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		Acyl, AsuIII, BbiII, BstACI, HgiGI, HgiHII, Hin1I, PamII
HgiAI Herpetosiphon giganteus HP1023
5' GWGCWC 3' CWCGWG
  5' ---GWGCW C--- 3'   3' ---C WCGWG--- 5'
Alw21I, AspHI, Bsh45I, BsiHKAI,
 Bsm6I, HpyF46II,  MspV281I
HgiBI Herpetosiphon giganteus Hpg5
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Psp03I , VpaK11AI
HgiCI Herpetosiphon giganteus Hpg9
5' GGYRCC 3' CCRYGG
  5' ---G GYRCC--- 3'   3' ---CCRYG G--- 5' 		AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiHI, MspB4I, PfaAI
HgiCII Herpetosiphon giganteus Hpg9
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme18I, BthAI, DsaIV, FspMSI, HgiHIII, Psp03I , VpaK11AI
HgiCIII Herpetosiphon giganteus Hpg9
5' GTCGAC 3' CAGCTG
  5' ---G TCGAC--- 3'   3' ---CAGCT G--- 5'
HgiDI Herpetosiphon giganteus Hpa2
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		Acyl, AsuIII, BbiII, BstACI, HgiGI, HgiI, Hin1I, PamII
HgiDII Herpetosiphon giganteus Hpa2
5' GTCGAC 3' CAGCTG
  5' ---G TCGAC--- 3'   3' ---CAGCT G--- 5'
HgiEI Herpetosiphon giganteus Hpg24
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme216I, BthAI, DsaIV, ErpI, FspMSI, HgiHIII, VpaK11AI
HgiGI Herpetosiphon giganteus Hpa1
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		Acyl, AosII, BbiII, BstACI, HgiI, HgiGI, Msp17I, PamII
HgiHI Herpetosiphon giganteus HP1049
5' GGYRCC 3' CCRYGG
  5' ---G GYRCC--- 3'   3' ---CCRYG G--- 5' 		AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiCI, MspB4I, PfaAI
HgiHII Herpetosiphon giganteus HP1049
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		AosII, AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Msp17I, PamII
HgiHIII Herpetosiphon giganteus HP1049
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme216I, DsaIV, Erpl, HgiBI, Psp03I , VpaK11AI , VpaK11BI
HgiJI Herpetosiphon giganteus HFS101
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I , VpaK11AI
HgiJII Herpetosiphon giganteus HFS101
5' GRGCYC 3' CYCGRG
  5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
HgiS22I Herpetosiphon giganteus
5' CCSGG 3' GGSCC
  5' ---CC SGG--- 3'   3' ---GGS CC--- 5' 		Ahal, AseII, AsuC2I, BcnI, CauII, Eco1831I , EcoHI , Kpn49kII
HhaI Haemophilus haemolyticus
5' GCGC 3' CGCG
  5' ---GCG C--- 3'   3' ---C GCG--- 5' 		AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, Hin6I , HinP1I , SciNI
Hhall Haemophilus haemolyticus
5' GANTC 3' CTNAG
  5' ---G ANTC--- 3'   3' ---CTNA G--- 5'
Hin1I Haemophilus influenzae RFL1
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		AcyI, AhaII, AosII, AstWI, AsuIII, HgiI, HgiDI, HgiDI, Hsp92I
Hin1II Haemophilus influenzae RFL1
5' CATG 3' GTAC
  5' ---CATG --- 3'   3' --- GTAC--- 5'
Hin2I Haemophilus influenzae RFL2
5' CCGG 3' GGCC
  5' ---C CGG--- 3'   3' ---GGC C--- 5'
Hin4I Haemophilus influenzae RFL4
5' GAYN 5 VTC 3' CTRN 5 VÄSKA
  5' ---GAYN 5 VTCN 7 NNNNNN --- 3'   3' ---CTRN 5 BAGN 7 N NNNNN--- 5'
Hin6I Haemophilus influenzae RFL6
5' GCGC 3' CGCG
  5' ---G CGC--- 3'   3' ---CGC G--- 5' 		AspLEI , BspLAI , BstHHI , CfoI , FnuDIII , HhaI , HsoI, HspAI, SciNI
HinJCI Haemophilus influenzae JC9
5' GTYRAC 3' CARYTG
  5' ---GTY RAC--- 3'   3' ---CAR YTG--- 5'
HinP1I 2FL3 Sök PDBe Haemophilus influenzae P1
5' GCGC 3' CGCG
  5' ---G CGC--- 3'   3' ---CGC G--- 5' 		BspLAI , BstHHI , CfoI , FnuDIII , Hhal , Hin6I, HsoI, HspAI, SciNI
HincII 2AUD Sök PDBe Haemophilus influenzae Rc
5' GTYRAC 3' CARYTG
  5' ---GTY RAC--- 3'   3' ---CAR YTG--- 5'
HindII Haemophilus influenzae Rd
5' GTYRAC 3' CARYTG
  5' ---GTY RAC--- 3'   3' ---CAR YTG--- 5'
HindIII 2E52 , Sök PDBe Haemophilus influenzae Rd
5' AAGCTT 3' TTCGAA
  5' ---A AGCTT--- 3'   3' ---TTCGA A--- 5'
HinfI Haemophilus influenzae Rf
5' GANTC 3' CTNAG
  5' ---G ANTC--- 3'   3' ---CTNA G--- 5'
HjaI Hyphomonas jannaschiana
5' GATATC 3' CTATAG
  5' ---GAT ATC--- 3'   3' ---CTA TAG--- 5'
HpaI Haemophilus parainfluenzae
5' GTTAAC 3' CAATTG
  5' ---GTT AAC--- 3'   3' ---CAA TTG--- 5'
HpaII Haemophilus parainfluenzae
5' CCGG 3' GGCC
  5' ---C CGG--- 3'   3' ---GGC C--- 5'
HphI Haemophilus parahaemolyticus
5' GGTGA 3' CCACT
  5' ---GGTGAN 6 NN --- 3'   3' ---CCACTN 6 N N--- 5' 		AsuHPI, SspD5I
Hpy8I Helicobacter pylori A 8-5
5' GTNNAC 3' CANNTG
  5' ---GTN NAC--- 3'   3' ---CAN NTG--- 5'
Hpy51I Helicobacter pylori 51
5' GTSAC 3' CASTG
  5' --- GTSAC--- 3'   3' ---CASTG --- 5'
Hpy99I 3GOX Helicobacter pylori J99
5' CGWCG 3' GCWGC
  5' ---CGWCG --- 3'   3' --- GCWGC--- 5'
Hpy178III Helicobacter pylori J178
5' TCNNGA 3' AGNNCT
  5' ---TC NNGA--- 3'   3' ---AGNN CT--- 5'
Hpy188I Helicobacter pylori J188
5' TCNGA 3' AGNCT
  5' ---TCN GA--- 3'   3' ---AG NCT--- 5'
Hpy188III Helicobacter pylori J188
5' TCNNGA 3' AGNNCT
  5' ---TC NNGA--- 3'   3' ---AGNN CT--- 5'
HpyAV Helicobacter pylori 26695
5' CCTTC 3' GGAAG
  5' ---CCTTCN 4 NN --- 3'   3' ---GGAAGN 4 N N--- 5'
HpyBI Helicobacter pylori Roberts
5' GTAC 3' CATG
  5' ---GT AC--- 3'   3' ---CA TG--- 5' 		AfaI, Csp6I , CviQI , CviRII , PabI , PlaAII, RsaI, RsaNI
HpyBII Helicobacter pylori Roberts
5' GTNNAC 3' CANNTG
  5' ---GTN NAC--- 3'   3' ---CAN NTG--- 5'
HpyCI Helicobacter pylori
5' GATATC 3' CTATAG
  5' ---GAT ATC--- 3'   3' ---CTA TAG--- 5'
HpyC1I Helicobacter pylori
5' CCATC 3' GGTAG
  5' ---CCATCNNNN N--- 3'   3' ---GGTAGNNNNN --- 5'
HpyCH4I Helicobacter pylori CH4
5' CATG 3' GTAC
  5' ---CATG --- 3'   3' --- GTAC--- 5'
HpyCH4III Helicobacter pylori CH4
5' ACNGT 3' TGNCA
  5' ---ACN GT--- 3'   3' ---TG NCA--- 5'
HpyCH4IV Helicobacter pylori CH4
5' ACGT 3' TGCA
  5' ---A CGT--- 3'   3' ---TGC A--- 5'
HpyCH4V Helicobacter pylori CH4
5' TGCA 3' ACGT
  5' ---TG CA--- 3'   3' ---AC GT--- 5'
HpyF10VI Helicobacter pylori RFL10
5' GCN 7 GC 3' CGN 7 CG
  5' ---GCNNNNN NNGC--- 3'   3' ---CGNN NNNNNCG--- 5'
HpyF44III Helicobacter pylori RFL44
5' TGCA 3' ACGT
  5' ---TG CA--- 3'   3' ---AC GT--- 5'
HsoI Haemophilus somnus 2336
5' GCGC 3' CGCG
  5' ---G CGC--- 3'   3' ---CGC G--- 5' 		AspLEI , BstHHI , CfoI , FnuDIII , Hin6I, HinP1I, HspAI, SciNI
Hsp92I Haemophilus sp. 92
5' GRCGYC 3' CYGCRG
  5' ---GR CGYC--- 3'   3' ---CYGC RG--- 5' 		AcyI, AhaII, AosII, AstWI, HgiI, HgiDI, HgiHII, Hin1I, Hsp92I
Hsp92II Haemophilus sp. 92
5' CATG 3' GTAC
  5' ---CATG --- 3'   3' --- GTAC--- 5'
HspAI Haemophilus sp. A
5' GCGC 3' CGCG
  5' ---G CGC--- 3'   3' ---CGC G--- 5' 		AspLEI , BspLAI , BstHHI , FnuDIII , Hhal , Hin6I, HinP1I, HsoI, SciNI
HsuI Haemophilus suis
5' AAGCTT 3' TTCGAA
  5' ---A AGCTT--- 3'   3' ---TTCGA A--- 5'

jag

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer
Italien Ilyobcater tartaricus
5' GCNGC 3' CGNCG
  5' ---GC NGC--- 3'   3' ---CGN CG--- 5'

K

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer
KasI Kluyvera ascorbata
5' GGCGCC 3' CCGCGG
  5' ---G GCGCC--- 3'   3' ---CCGCG G--- 5'
Kaz48kI Klebsiella azeanae
5' RGGNCCY 3' YCCNGGR
  5' ---RGGNC CY--- 3'   3' ---YC CNGGR--- 5'
KoxII Klebsiella oxytoca
5' GRGCYC 3' CYCGRG
  5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
KpnI Klebsiella pneumoniae OK8
5' GGTACC 3' CCATGG
  5' ---GGTAC C--- 3'   3' ---C CATGG--- 5' 		Acc65I , AhaB8I , Asp718I , SthI
Kpn2I Klebsiella pneumoniae RFL2
5' TCCGGA 3' AGGCCT
  5' ---T CCGGA--- 3'   3' ---AGGCC T--- 5' 		Aor13HI, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
Kpn378I Klebsiella pneumoniae 378
5' CCGCGG 3' GGCGCC
  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
Kpn2kI Klebsiella pneumoniae 2k
5' CCNGG 3' GGNCC
  5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
Kpn49kI Klebsiella pneumoniae 49k
5' GAATTC 3' CTAAG
  5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
Kpn49kII Klebsiella pneumoniae 49k
5' CCSGG 3' GGSCC
  5' --- CCSGG--- 3'   3' ---GGSCC --- 5' 		Ahal , AsuC2I , BcnI , CauII , EcoHI, HgiS22I , Mgl14481I , NciI ,
KspI Kluyvera sp.
5' CCGCGG 3' GGCGCC
  5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
Ksp22I Kurthia sp. 22
5' TGATCA 3' ACTAGT
  5' ---T GATCA--- 3'   3' ---ACTAG T--- 5'
AbaI, BclI, BsiQI,
BspXII, BstT7I, Fbal,  ParI 
Ksp632I Kluyvera sp. 632
5' CTCTTC 3' GAGAAG
  5' ---CTCTTCN NNN--- 3'   3' ---GAGAAGNNNN --- 5'
KspAI Kurthia sp. N88
5' GTTAAC 3' CAATTG
  5' ---GTT AAC--- 3'   3' ---CAA TTG--- 5'
Kzo9I Kurthia zopfii 9
5' GATC 3' CTAG
  5' --- GATC--- 3'   3' ---CTAG --- 5' 		Bce243I, Bsp105I, BspFI, BstMBI, CpfI, LlaAI, NdeII, Sth368I
Kzo49I Kurthia zopfii 49
5' GGWCC 3' CCWGG
  5' ---G GWCC--- 3'   3' ---CCWG G--- 5' 		Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I , VpaK11AI

Anteckningar

Hämtad från " https://en.wikipedia.org/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_G–K&oldid=1134675692 "