Lista över skärplatser för restriktionsenzym: E–F

Huvudartiklar: Lista över skärplatser för restriktionsenzym och restriktionsenzym

Legenden om nukleobaser
Koda	Nukleotid representerad
A	Adenin (A)
C	Cytosin (C)
G	Guanin (G)
T	Tymin (T)
N	A, C, G eller T
M	A eller C
R	A eller G
W	A eller T
Y	C eller T
S	C eller G
K	G eller T
H	A, C eller T
B	C, G eller T
V	A, C eller G
D	A, G eller T

Den här artikeln innehåller en lista över de mest studerade restriktionsenzymer vars namn börjar med E till F inklusive. Den innehåller cirka 110 enzymer.

Följande information ges:

• Enzym : Accepterat namn på molekylen , enligt den internationellt antagna nomenklaturen och bibliografiska referenser . (Mer läsning: se avsnittet " Nomenklatur " i artikeln " Restriktionsenzym ".)
• PDB-kod : Kod som används för att identifiera strukturen av ett protein i PDB -databasen över proteinstrukturer. 3D-atomstrukturen hos ett protein ger mycket värdefull information för att förstå de intima detaljerna i dess verkningsmekanism.
• Källa : Organism som naturligt producerar enzymet.
• Igenkänningssekvens : Sekvens av DNA som känns igen av enzymet och till vilken det specifikt binder.
• Skärning : Skärställe och DNA-produkter från snittet. Igenkänningssekvensen dussintals nukleotider bort från igenkänningsstället.
• Isoschizomerer och neoschizomerer : En isoschizomer är ett enzym som känner igen samma sekvens som ett annat. En neoschizomer är en speciell typ av isoschizomer som känner igen samma sekvens som en annan, men skär på ett annat sätt. Ett maximalt antal av 8-10 vanligaste isoschizomerer är indikerade för varje enzym men det kan finnas många fler. Neoschizomerer visas med fet och grön typsnitt (t.ex.: BamHI ). När "Ingen på datum " anges betyder det att det inte fanns några registrerade isoschizomerer i databaserna det datumet med en tydligt definierad skärplats. Isoschizomerer indikerade med vitt teckensnitt och grå bakgrund motsvarar enzymer som inte finns med i de aktuella listorna:

som i detta ej listade enzym:

EcoR70I

Restriktionsenzymer

E

Enzym	PDB-kod	Källa	Igenkänningssekvens	Skära	Isoschizomerer
EacI		Eubacterium acidaminophilum	5' GGATC 3' CCTAG	5' ---GGATCNNNN N--- 3'   3' ---CCTAGNNNNN --- 5'	AclWI, AlwI, BinI,  BpuFI,  BspPI, BstH9I,  Bst31TI, Bth617I, Ral8I
EaeI		Enterobacter aerogenes	5' YGGCCR 3' RCCGGY	5' ---Y GGCCR--- 3'   3' ---RCCGG Y--- 5'
Eae46I		Enterobacter aerogenes 46	5' CCGCGG 3' GGCGCC	5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
EaeAI		Enterobacter aerogenes	5' CCCGGG 3' GGGCCC	5' ---C CCGGG--- 3'   3' ---GGGCC C--- 5'	AhyI, Cfr9I, EclRI, PaeBI , PspAI, PspALI , XcyI, XmaI, XmaCI
EagI		Enterobacter agglomerans	5' CGGCCG 3' GCCGGC	5' ---C GGCCG--- 3'   3' ---GCCGG C--- 5'	AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIII
EagBI		Enterobacter agglomerans CBNU45	5' CGATCG 3' GCTAGC	5' ---CGAT CG--- 3'   3' ---GC TAGC--- 5'	Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorII
EagMI		Enterobacter agglomerans M3	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI
Eam1104I		Enterobacter amnigenus RFL1104	5' CTCTTC 3' GAGAAG	5' ---CTCTTCN NNN--- 3'   3' ---GAGAAGNNNN --- 5'
Eam1105I		Enterobacter amnigenus RFL1105	5' GACN 5 GTC 3' CTGN 5 CAG	5' ---GACNNN NNGTC--- 3'   3' ---CTGNN NNNCAG--- 5'	AhdI, AspEI,  BmeRI,  BspOVI, DriI, EclHKI, NruGI
EarI		Enterobacter aerogenes	5' CTCTTC 3' GAGAAG	5' ---CTCTTCN NNN--- 3'   3' ---GAGAAGNNNN --- 5'
EcaI		Enterobacter cloacae	5' GGTNACC 3' CCANTGG	5' ---G GTNACC--- 3'   3' ---CCANTG G--- 5'	AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I
EciI		Escherichia coli	5' GGCGGA 3' CCGCCT	5' ---GGCGGAN 8 NNN --- 3'   3' ---CCGCCTN 8 N NN--- 5'
Eci125I		Escherichia coli VKM-125	5' GGTNACC 3' CCANTGG	5' ---G GTNACC--- 3'   3' ---CCANTG G--- 5'	AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I
EclI		Enterobacter cloacae	5' CAGCTG 3' GTCGAC	5' ---CAG CTG--- 3'   3' ---GTC GAC--- 5'
Ecl136II		Enterobacter cloacae RFL136	5' GAGCTC 3' CTCGAG	5' ---GAG CTC--- 3'   3' ---CTC GAG--- 5'
EclHKI		Enterobacter cloacae	5' GACN 5 GTC 3' CTGN 5 CAG	5' ---GACNNN NNGTC--- 3'   3' ---CTGNN NNNCAG--- 5'	AhdI, AspEI,  BmeRI,  BspOVI, DriI, Eam1105I, NruGI
EclRI		Enterobacter cloacae R	5' CCCGGG 3' GGGCCC	5' ---C CCGGG--- 3'   3' ---GGGCC C--- 5'	AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI , XcyI, XmaI, XmaCI
EclXI		Enterobacter cloacae 590	5' CGGCCG 3' GCCGGC	5' ---C GGCCG--- 3'   3' ---GCCGG C--- 5'	AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIII
Ecl18kI	2FQZ	Enterobacter cloacae 18k	5' CCNGG 3' GGNCC	5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
Ecl37kI		Enterobacter cloacae 37k	5' CTGCAG 3' GACGTC	5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'	AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenI
Ecl2zI		Enterobacter cloacae 2z	5' CTGCAG 3' GACGTC	5' ---CTGCA G--- 3'   3' ---G ACGTC--- 5'	AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenI
Eco24I		Escherichia coli RFL24	5' GRGCYC 3' CYCGRG	5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
Eco31I		Escherichia coli RFL31	5' GGTCTC 3' CCAGAG	5' ---GGTTCN NNNN--- 3'   3' ---CCAGAGNNNNNN --- 5'
Eco32I		Escherichia coli RFL32	5' GATATC 3' CTATAG	5' ---GAT ATC--- 3'   3' ---CTA TAG--- 5'
Eco47I		Escherichia coli RFL47	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI
Eco47III		Escherichia coli RFL47	5' AGCGCT 3' TCGCGA	5' ---AGC GCT--- 3'   3' ---TCG CGA--- 5'	AfeI, AitI, Aor51H, FunI
Eco52I		Escherichia coli RFL52	5' CGGCCG 3' GCCGGC	5' ---C GGCCG--- 3'   3' ---GCCGG C--- 5'	AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIII
Eco56I		Escherichia coli RFL56	5' GCCGGC 3' CGGCCG	5' ---G CCGGC--- 3'   3' ---CGGCC G--- 5'
Eco57I		Escherichia coli RFL57	5' CTGAAG 3' GACTTC	5' ---CTGAAGN 12 NNNN --- 3'   3' ---GACTTCN 12 NN NN--- 5'	Acul, BspKT5I
Eco64I		Escherichia coli RFL64	5' GGYRCC 3' CCRYGG	5' ---G GYRCC--- 3'   3' ---CCRYG G--- 5'	BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAI
Eco72I		Escherichia coli RFL72	5' CACGTG 3' GTGCAC	5' ---CAC GTG--- 3'   3' ---GTG CAC--- 5'	AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCI
Eco78I		Escherichia coli RFL78	5' GGCGCC 3' CCGCGG	5' ---GGC GCC--- 3'   3' ---CCG CGG--- 5'
Eco81I		Escherichia coli RFL81	5' CCTNAGG 3' GGANTCC	5' ---CC TNAGG--- 3'   3' ---GGANT CC--- 5'	AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Eco88I		Escherichia coli RFL88	5' CYCGRG 3' GRGCYC	5' ---C YCGRG--- 3'   3' ---GRGCY C--- 5'	AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAI
Eco91I		Escherichia coli RFL91	5' GGTNACC 3' CCANTGG	5' ---G GTNACC--- 3'   3' ---CCANTG G--- 5'	Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAII
Eco105I		Escherichia coli RFL105	5' TACGTA 3' ATGCAT	5' ---TAC GTA--- 3'   3' ---ATG CAT--- 5'
Eco130I		Escherichia coli RFL130	5' CCWWGG 3' GGWWCC	5' ---C CWWGG--- 3'   3' ---GGWWC C--- 5'
Eco147I		Escherichia coli RFL147	5' AGGCCT 3' TCCGGA	5' ---AGG CCT--- 3'   3' ---TCC GGA--- 5'	AatI, AspMI, GdiI, Pcel, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI
Eco255I		Escherichia coli RFL255	5' AGTACT 3' TCATGA	5' ---AGT ACT--- 3'   3' ---TCA TGA--- 5'	Acc113I, AssI,  BmcAI, Bpa34I,  Dpal, RflFII, Scal, Zrml
Eco1831I		Escherichia coli RFL1831	5' CCSGG 3' GGSCC	5' --- CCSGG--- 3'   3' ---GGSCC --- 5'	Ahal , AseII , AsuC2I , BcnI ,   BpuMI ,   CauII , EcoHI, HgiS22I
EcoA4I		Escherichia coli A4	5' GGTCTC 3' CCAGAG	5' ---GGTTCN NNNN--- 3'   3' ---CCAGAGNNNNN --- 5'
EcoHI		Escherichia coli HI	5' CCSGG 3' GGSCC	5' --- CCSGG--- 3'   3' ---GGSCC --- 5'	AsuC2I , BcnI , BpuMI , CauII , EcoHI, HgiS22I , Kpn49kII, NciI
EcoHK31I		Escherichia coli HK31	5' YGGCCR 3' RCCGGY	5' ---Y GGCCR--- 3'   3' ---RCCGG Y--- 5'
EcoICRI		Escherichia coli 2bT	5' GAGCTC 3' CTCGAG	5' ---GAG CTC--- 3'   3' ---CTC GAG--- 5'
Eco75KI		Escherichia coli BKM	5' GRGCYC 3' CYCGRG	5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
Eco57MI		Escherichia coli RFL57M	5' CTGRAG 3' GACYTC	5' ---CTGRAGN 12 NNNN --- 3'   3' ---GACYTCN 12 NN NN--- 5'
EcoNI		Escherichia coli	5' CCTN 5 AGG 3' GGAN 5 TCC	5' ---CCTNN NNNAGG--- 3'   3' ---GGANNN NNTCC--- 5'
EcoO44I		Escherichia coli O44 Hiromi	5' GGTCTC 3' CCAGAG	5' ---GGTTCN NNNN--- 3'   3' ---CCAGAGNNNNN --- 5'
EcoO65I		Escherichia coli Klla	5' GGTNACC 3' CCANTGG	5' ---G GTNACC--- 3'   3' ---CCANTG G--- 5'	AcrII, Bse64I, BstEII, BstPI, BstT9I, Ecal, Eco91I, EcoO128I
EcoO109I	1WTD	Escherichia coli H709c	5' RGGNCCY 3' YCCNGGR	5' ---RG GNCCY--- 3'   3' ---YCCNG GR--- 5'
EcoO128I		Escherichia coli O128Ly3	5' GGTNACC 3' CCANTGG	5' ---G GTNACC--- 3'   3' ---CCANTG G--- 5'	AspAI, Bse64I, BstEII, BstT9I, Ecal, EcoO65I, NspSAII, PspEI
EcoP15I		Escherichia coli P15	5' CAGCAG 3' GTCGTC	5' ---CAGCAGN 25 NN --- 3'   3' ---GTCGTCN 25 NN--- 5'
EcoR124II	, Sök	Escherichia coli	5' 5'-GAAN 7 RTCG-3'	5'
EcoRI	1QC9 , Sök efter EcoR1-strukturer	Escherichia coli RY13	5' GAATTC 3' CTAAG	5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'
EcoRII	1NA6	Escherichia coli R245	5' CCWGG 3' GGWCC	5' --- CCWGG--- 3'   3' ---GGWCC --- 5'	ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI , ZanI
EcoRV	1RVE Sök EcoRV	Escherichia coli J62 pLG74	5' GATATC 3' CTATAG	5' ---GAT ATC--- 3'   3' ---CTA TAG--- 5'
EcoT14I		Escherichia coli TB14	5' CCWWGG 3' GGWWCC	5' ---C CWWGG--- 3'   3' ---GGWWC C--- 5'
EcoT22I		Escherichia coli TB22	5' ATGCAT 3' TACGTA	5' ---ATGCA T--- 3'   3' ---T ACGTA--- 5'	BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5II
EcoT38I		Escherichia coli TH38	5' GRGCYC 3' CYCGRG	5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
EcoVIII		Escherichia coli E1585-68	5' AAGCTT 3' TTCGAA	5' ---A AGCTT--- 3'   3' ---TTCGA A--- 5'
Eco13kI		Escherichia coli 13k	5' CCNGG 3' GGNCC	5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
Eco21kI		Escherichia coli 21k	5' CCNGG 3' GGNCC	5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
Eco27kI		Escherichia coli 27	5' CYCGRG 3' GRGCYC	5' ---C YCGRG--- 3'   3' ---GRGCY C--- 5'	Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAI
Eco29kI		Escherichia coli	5' CCGCGG 3' GGCGCC	5' ---CCGC GG--- 3'   3' ---GG CGCC--- 5'
Eco53kI		Escherichia coli 53k	5' GAGCTC 3' CTCGAG	5' ---GAG CTC--- 3'   3' ---CTC GAG--- 5'
Eco137kI		Escherichia coli 137k	5' CCNGG 3' GGNCC	5' --- CCNGG--- 3'   3' ---GGNCC --- 5'
Egel		Enterobacter gergoviae NA	5' GGCGCC 3' CCGCGG	5' ---GGC GCC--- 3'   3' ---CCG CGG--- 5'
EheI		Erwinia herbicola 9/5	5' GGCGCC 3' CCGCGG	5' ---GGC GCC--- 3'   3' ---CCG CGG--- 5'
ErhI		Erwinia rhaponici B9	5' CCWWGG 3' GGWWCC	5' ---C CWWGG--- 3'   3' ---GGWWC C--- 5'
ErhB9I		Erwinia rhaponici B9	5' CGATCG 3' GCTAGC	5' ---CGAT CG--- 3'   3' ---GC TAGC--- 5'	Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorII
ErhB9II		Erwinia rhaponici B9	5' CCWWGG 3' GGWWCC	5' ---C CWWGG--- 3'   3' ---GGWWC C--- 5'
Erpl		Erwinia rhaponici	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI
EsaBC3I		Miljöprov BC3	5' TCGA 3' AGCT	5' ---TC GA--- 3'   3' ---AG CT--- 5'
EsaBC4I		Miljöprov BC4	5' GGCC 3' CCGG	5' ---GG CC--- 3'   3' ---CC GG--- 5'
EspI		Eucapsis sp.	5' GCTNAGC 3' CGANTCG	5' ---GC TNAGC--- 3'   3' ---CGANT CG--- 5'
Esp3I		Erwinia sp. RFL3	5' CGTCTC 3' GCAGAG	5' ---CGTCTCN NNNN--- 3'   3' ---GCAGAGNNNNN --- 5'
Esp4I		Erwinia sp. RFL4	5' CTAAG 3' GAATTC	5' ---C TTAAG--- 3'   3' ---GAATT C--- 5'
Esp1396I		Enterobacter sp. RFL1396	5' CCAN 5 TGG 3' GGTN 5 ACC	5' ---CCANNN NTGG--- 3'   3' ---GGTN NNNNACC--- 5'	AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I

F

Enzym	PDB-kod	Källa	Igenkänningssekvens	Skära	Isoschizomerer
FalI		Flavobacterium aquatile Ob10	5' AAGN 5 CTT 3' TTCN 5 GAA	5' ---AAGN 5 CTTN 7 NNNNNN --- 3'   3' ---TTCN 5 GAAN 7 N NNNNN--- 5'
FalII		Flavobacterium aquatile Ob10	5' CGCG 3' GCGC	5' ---CG CG--- 3'   3' ---GC GC--- 5'	BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI , ThaI
FaqI		Flavobacterium aquatile RFL1	5' GGGAC 3' CCCTG	5' ---GGGACN 8 NN NNNN--- 3'   3' ---CCCTGN 8 NNNNNN --- 5'
FatI		Flavobacterium aquatile NL3	5' CATG 3' GTAC	5' --- CATG--- 3'   3' ---GTAC --- 5'
FauI		Flavobacterium aquatile	5' CCCGC 3' GGGCG	5' ---CCCGCNNNN NN--- 3'   3' ---GGGCGNNNNNN --- 5'
FauBII		Flavobacterium aureus B	5' CGCG 3' GCGC	5' ---CG CG--- 3'   3' ---GC GC--- 5'	Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI , ThaI
FauNDI		Flavobacterium aquatile ND	5' CATATG 3' GTATAC	5' ---CA TATG--- 3'   3' ---GTAT AC--- 5'
FbaI		Flavobacterium balustinum	5' TGATCA 3' ACTAGT	5' ---T GATCA--- 3'   3' ---ACTAG T--- 5'	AbaI, BclI, BsiQI, BspXII, BstT7I, Ksp22I,  ParI
FblI		Flavobacterium balustinum	5' GTMKAC 3' CAKMTG	5' ---GT MKAC--- 3'   3' ---CAKM TG--- 5'	AccI, XmiI
FbrI		Flavobacterium breve	5' GCNGC 3' CGNCG	5' ---GC NGC--- 3'   3' ---CGN CG--- 5'
FdiI		Fremyella diplosiphon	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BthAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI
FdiII		Fremyella diplosiphon	5' TGCGCA 3' ACGCGT	5' ---TGC GCA--- 3'   3' ---ACG CGT--- 5'	Acc16I, AosI, AviII, FspI, MstI, NsbI, PamI, Pun14627I
FgoI		Fervidobacterium gondwanense AB39T	5' CTAG 3' GATC	5' ---C TAG--- 3'   3' ---GAT C--- 5'
FmuI		Flavobacterium multivorum	5' GGNCC 3' CCNGG	5' ---GGNC C--- 3'   3' ---C CNGG--- 5'	FmuI AvcI , BavAII , Bce22I , Bsu54I , NspIV , Pde12I , UnbI
FnuAI		Fusobacterium nucleatum A	5' GANTC 3' CTNAG	5' ---G ANTC--- 3'   3' ---CTNA G--- 5'
FnuCI		Fusobacterium nucleatum C	5' GATC 3' CTAG	5' --- GATC--- 3'   3' ---CTAG --- 5'	Bme12I, Bsp67I, Bst19II, CcyI, FnuEI, MgoI, NphI, SauMI
FnuDI		Fusobacterium nucleatum D	5' GGCC 3' CCGG	5' ---GG CC--- 3'   3' ---CC GG--- 5'
FnuDII		Fusobacterium nucleatum D	5' CGCG 3' GCGC	5' ---CG CG--- 3'   3' ---GC GC--- 5'	Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FnuDII, Thai
FnuDIII		Fusobacterium nucleatum D	5' GCGC 3' CGCG	5' ---GCG C--- 3'   3' ---C GCG--- 5'	AspLEI, BspLAI, BstHHI, CfoI, HhaI, Hin6I , HinP1I , HspAI , SciNI
FnuEI		Fusobacterium nucleatum E	5' GATC 3' CTAG	5' --- GATC--- 3'   3' ---CTAG --- 5'	Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, Sau3AI
Fnu4HI		Fusobacterium nucleatum 4H	5' GCNGC 3' CGNCG	5' ---GC NGC--- 3'   3' ---CGN CG--- 5'
FokI	2FOK	Flavobacterium okeanokoites	5' GGATG 3' CCTAC	5' ---GGATGN 8 N NNNN--- 3'   3' ---CCTACN 8 NNNNN --- 5'
FriOI		Flavobacterium sp. O9	5' GRGCYC 3' CYCGRG	5' ---GRGCY C--- 3'   3' ---C YCGRG--- 5'
FseI		Frankia sp. Eullb	5' GGCCGGCC 3' CCGGCCGG	5' ---GGCCGG CC--- 3'   3' ---CC GGCCGG--- 5'
FsiI		Frankia sp.	5' RAATTY 3' YTTAAR	5' ---R AATTY--- 3'   3' ---YTTAA R--- 5'	AcsI, ApoI,  CfaI,  XapI
FspI		Fischerella sp.	5' TGCGCA 3' ACGCGT	5' ---TGC GCA--- 3'   3' ---ACG CGT--- 5'	Acc16I, AosI, AviII, FdII, MstI, NsbI, PamI, Pun14627I
FspII		Fischerella sp.	5' TTCGAA 3' AAGCTT	5' ---TT CGAA--- 3'   3' ---AAGC TT--- 5'	Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, Ssp1I, SviI
Fsp1604I		Flavobacterium sp. I 16-04	5' CCWGG 3' GGWCC	5' ---CC WGG--- 3'   3' ---GGW CC--- 5'	ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII , SleI
FspAI		Flexibacter sp. TV-m21K	5' RTGCGCAY 3' YACGCGTR	5' ---RTGC GCAY--- 3'   3' ---YACG CGTR--- 5'
FspBI		Flavobacterium sp. RFLI	5' CTAG 3' GATC	5' ---C TAG--- 3'   3' ---GAT C--- 5'
Fsp4HI		Flavobacterium sp. 4H	5' GCNGC 3' CGNCG	5' ---GC NGC--- 3'   3' ---CGN CG--- 5'
FspMSI		Fischerella sp.	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BthAI, DsaIV, ErpI, HgiBI, HgiEI, Kzo49I, SmuEI
FssI		Fibrobacter succinogenes S85	5' GGWCC 3' CCWGG	5' ---G GWCC--- 3'   3' ---CCWG G--- 5'	Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Kzo49I, VpaK11AI
Kul		Fischerella uniformis	5' AGCGCT 3' TCGCGA	5' ---AGC GCT--- 3'   3' ---TCG CGA--- 5'	AfeI, AitI, Aor51H, Eco47III
Kul II		Fischerella uniformis	5' GAATTC 3' CTAAG	5' ---G AATTC--- 3'   3' ---CTTAA G--- 5'