Lista över skärplatser för restriktionsenzym: O–R

Legenden om nukleobaser
Koda	Nukleotid representerad
A	Adenin (A)
C	Cytosin (C)
G	Guanin (G)
T	Tymin (T)
N	A, C, G eller T
M	A eller C
R	A eller G
W	A eller T
Y	C eller T
S	C eller G
K	G eller T
H	A, C eller T
B	C, G eller T
V	A, C eller G
D	A, G eller T

Den här artikeln innehåller en lista över de mest studerade restriktionsenzymer vars namn börjar med O till och med R. Den innehåller cirka 130 enzymer. Följande information ges:

Enzym : Accepterat namn på molekylen , enligt den internationellt antagna nomenklaturen och bibliografiska referenser . (Mer läsning: se avsnittet " Nomenklatur " i artikeln " Restriktionsenzym ".)
PDB-kod : Kod som används för att identifiera strukturen av ett protein i PDB -databasen över proteinstrukturer. 3D-atomstrukturen hos ett protein ger mycket värdefull information för att förstå de intima detaljerna i dess verkningsmekanism.
Källa : Organism som naturligt producerar enzymet.
Igenkänningssekvens : Sekvens av DNA som känns igen av enzymet och till vilken det specifikt binder.
Skärning : Skärställe och DNA-produkter från snittet. Igenkänningssekvensen dussintals nukleotider bort från igenkänningsstället.
Isoschizomerer och neoschizomerer : En isoschizomer är ett enzym som känner igen samma sekvens som ett annat. En neoschizomer är en speciell typ av isoschizomer som känner igen samma sekvens som en annan, men skär på ett annat sätt. Ett maximalt antal av 8-10 vanligaste isoschizomerer är indikerade för varje enzym men det kan finnas många fler. Neoschizomerer visas med fet och grön typsnitt (t.ex.: BamHI ). När "Ingen på datum " anges betyder det att det inte fanns några registrerade isoschizomerer i databaserna det datumet med en tydligt definierad skärplats. Isoschizomerer indikerade med vitt teckensnitt och grå bakgrund motsvarar enzymer som inte finns med i de aktuella listorna:

som i detta ej listade enzym:

EcoR70I

Hela listans navigering

Restriktionsenzymer

O

Enzym	PDB-kod	Källa	Igenkänningssekvens	Skära	Isoschizomerer
OfoI		Oscillatoria foreaui A-1340	`5' CYCGRG` `3' GRGCYC`	`5' ---C YCGRG--- 3'` `3' ---GRGCY C--- 5'`	AquI, Aval, Bse15I, BspLU4I, Eco27kI, Nli3877I , PlaAI, PunAI
OkrAI		Oceanospirillum kriegii	`5' GGATCC` `3' CCTAGG`	`5' ---G GATCC--- 3'` `3' ---CCTAG G--- 5'`	BamHI , Bce751I, BnaI, Bsp98I, BstI, OkrAI, SolI, Uba4009I
OliI		Oceanospirillum linum 4-5D	`5' CACN ₄ GTG` `3' GTGN ₄ CAC`	`5' ---CACNN NNGTG--- 3'` `3' ---GTGNN NNCAC--- 5'`	AleI
OxaNI		Oerskovia xanthineolytica N	`5' CCTNAGG` `3' GGANTCC`	`5' ---CC TNAGG--- 3'` `3' ---GGANT CC--- 5'`	AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

P

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer

PabI

2DVY

Pyrococcus abyssi

5' GTAC 3' CATG

5' ---GTA C--- 3' 3' ---C ATG--- 5'

AfaI , Csp6I, CviQI, HpyBI ,
HpyBI, PlaAII , RsaI ,	RsaNI ,

Pacl Pseudomonas alcaligenes 5' TTAATTAA 3' AATTAATT 5' ---TTAAT TAA--- 3' 3' ---AAT TAATT--- 5'

Pac25I Pseudomonas alcaligenes 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspAI, PspALI , XcyI, XmaI, XmaCI

PaeI Pseudomonas aeruginosa 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5'

PaeAI Pseudomonas aeruginosa 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'

PaeBI Pseudomonas aeruginosa - 18 5' CCCGGG 3' GGGCCC 5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI , SmaI , TspMI, XcyI, XmaI, XmaCI

PaeHI Pseudomonas aeruginosa 4148 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'

PaeHII Pseudomonas aeruginosa 4148 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII, Srl5DI, Sst12I, XcpI

PaeQI Pseudomonas aeruginosa Q2 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'

PaeR7I Pseudomonas aeruginosa PA0303 pMG7 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI

Pae2kI Pseudomonas aeruginosa 2k 5' AGATCT 3' TTCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5'

Pae5kI Pseudomonas aeruginosa 5k 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'

Pae14kI Pseudomonas aeruginosa 14k 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'

Pae17kI Pseudomonas aeruginosa 17k 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'

Pae18kI Pseudomonas aeruginosa 18k 5' AGATCT 3' TTCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5'

PagI Pseudomonas alcaligenes Sau 14-027 5' TCATGA 3' AGTACT 5' ---T CATGA--- 3' 3' ---AGTAC T--- 5'

PalI Providencia alcalifaciens 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

PamI Phormidium ambiguum 5' TGCGCA 3' ACGCGT 5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdII, FspI, MstI, NsbI, Pun14627I

PamII Phormidium ambiguum 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AsuIII, BsaHI, BstACI, HgiDI, HgiGI, HgiHII, Hin1I, Hsp92I, Msp17I

PanI Pseudomonas alkanolytica 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

PasI Pseudomonas anguilliseptica RFL1 5' CCCWGGG 3' GGGWCCC 5' ---CC CWGGG--- 3' 3' ---GGGWC CC--- 5' — Inga i maj 2010 —

Paul Paracoccus alcaliphilus ZVK3-3 5' GCGCGC 3' CGCGCG 5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5'

PauAI Phormidium autumnale 5' RCATGY 3' YGTACR 5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5'

PauAII Phormidium autumnale 5' TTTAAA 3' AAATTT 5' ---TTT AAA--- 3' 3' ---AAA TTT--- 5' AhaIII, DraI, SruI

PCeI Planococcus citreus 55 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, GdiI, Pcel, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI

PciI Planococcus citreus SE-F45 5' ACATGT 3' TGTACA 5' ---A CATGT--- 3' 3' ---TGTAC A--- 5'

PctI

Planococcus citreus SM

5' GAATGC 3' CTTACG

5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5'

Asp26HI, Asp27HI, Asp35HI
BmaHI,	BsaMI, BscCI, Mva1269I

Pde12I Paracoccus denitrificans 12 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI

Pde133I Paracoccus denitrificans 133 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

Pde137I Paracoccus denitrificans 137 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5'

PdiI Pseudomonas diminuta Mck 33-321 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'

PdmI Pseudomonas diminuta Auk 5-324 5' GAAN ₄ TTC 3' CTTN ₄ AAG 5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, MroXI, XmnI

PfaAI Phormidium favosum 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BbvBI, BshNI, Eco64I, HgiCI, HgiHI, MspB4I

PfaAII Phormidium favosum 5' CATATG 3' GTATAC 5' ---CA TATG--- 3' 3' ---GTAT AC--- 5'

PfaAIII Phormidium favosum 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5'

PfeI Psychrobacter faecalis RFL1 5' GAWTC 3' CTWAG 5' ---G AWTC--- 3' 3' ---CTWA G--- 5'

Pfl8I Pseudomonas fluorescens 8 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AliAJI, BspBI, BsuBI, CflI, CfrA4I, PaePI, Pfl21I, PstI, SflII, Sst12I

Pfl21I Pseudomonas sp. 21 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'

Pfl23II Pseudomonas fluorescens RFL23 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5'

Pfl27I Pseudomonas fluorescens RFL27 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5'

PflBI Pseudomonas fluorescens typ B 5' CCAN ₅ TGG 3' GGTN ₅ ACC 5' ---CCANNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflMI, Van91I

PflFI Pseudomonas fluorescens biotyp F 5' GACNNNGTC 3' CTGNNNCAG 5' ---GACN NNGTC--- 3' 3' ---CTGNN NCAG--- 5' AspI, AtsI, PsyI, TelI, Tth111I

PflKI Pseudomonas fluorescens 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

PflMI Pseudomonas fluorescens 5' CCAN ₅ TGG 3' GGTN ₅ ACC 5' ---CCANNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, Van91I

PfoI Pseudomonas fluorescens biovar 126 5' TCCNGGA 3' AGGNCCT 5' ---T CCNGGA--- 3' 3' ---AGGNCC T--- 5'

Pgal Pseudomonas gladioli B4 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BbvAII, BliAI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I

PhaI Pasteurella haemolytica 5' GCATC 3' CGTAG 5' ---GCATCN ₄ N NNNN--- 3' 3' ---CGTAGN ₄ NNNNN --- 5'

PhoI Pyrococcus horikoshii OT3 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

PinAI

Pseudomonas inequalis

5' ACCGGT 3' TGGCCA

5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5'

AgeI, AsiaAI,	AsiGI,	BshTI
CsiAI, CspAI

PinBI Phormidium inundatum 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' EcoT22I, BfrBI, Mph1103I, NsiI, Ppu10I , SepI, SspD5II, Zsp2I

PinBII Phormidium inundatum 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' Aor13HI, BlfI, Bsp13I, BspEI, Bsu23I, Kpn2I, PinBII, Ptal

PlaI Phormidium lapideum 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

PlaII Phormidium lapideum 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, SfuI, SspRFI

PlaAI Phormidium laminosum 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco27kI, Nli3877I , OfoI, PunAI

PlaAII

Phormidium laminosum

5' GTAC 3' CATG

5' ---GT AC--- 3' 3' ---CA TG--- 5'

AfaI, Csp6I , CviQI , CviRII ,
HpyBI, PabI , RsaI,	RsaNI ,

PleI Pseudomonas lemoignei 5' GAGTC 3' CTCAG 5' ---GAGTCNNNN N--- 3' 3' ---CTCAGNNNNN --- 5'

Ple19I Pseudomonas lemoignei 19 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa22MI, BspCI, ErhB9I, MvrI, Psu161I, PvuI, RshI, XorII

PmaCI Pseudomonas maltophilia CB50P 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmlI, PspCI

PmeI Pseudomonas mendocina 5' GTTTAAAC 3' CAAATTTG 5' ---GTTT AAAC--- 3' 3' ---CAAA TTTG--- 5' MssI

Pme55I Pseudomonas mendocina 55 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, Pcel, SarI, Sru30DI, SseBI, SteI, StuI

PmlI Pseudomonas maltophilia 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PspCI

PovII Proteus vulgaris 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'

PpaAI Phormidium papyraceum 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, SfuI, SspRFI, SviI

PpaAII Phormidium papyraceum 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5'

Ppel Phormidium persicinum 5' GGGCCC 3' CCCGGG 5' ---GGGCC C--- 3' 3' ---C CCGGG--- 5' Apal, Bsp120I , PspOMI

PpiI Pseudomonas putida Jo 4-731 5' GAACN ₅ CTC 3' CTTGN ₅ GAG 5' ---GAACN ₅ CTCN ₇ NNNNNN --- 3' 3' ---CTTGN ₅ GAGN ₇ N NNNNN--- 5'

PpsI Pseudomonas pseudoalcaligenes PL 5' GAGTC 3' CTCAG 5' ---GAGTCNNNN N--- 3' 3' ---CTCAGNNNNN --- 5'

Ppu10I Pseudomonas putida RFL10 5' ATGCAT 3' TACGTA 5' ---A TGCAT--- 3' 3' ---TACGT A--- 5' BfrBI , Csp68KII , EcoT22I , Mph1103I , NsiI , SepI , SspD5II

Ppu111I Pseudomonas putida P111 5' GAATTC 3' CTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'

PpuAI Pseudomonas putida A1 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5'

PpuMI Pseudomonas putida M 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5'

PpuXI Pseudomonas putida X 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5'

PshAI Plesiomonas shigelloides 319-73 5' GACN ₄ GTC 3' CTGN ₄ CAG 5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5'

PshBI Plesiomonas shigelloides TPS970 5' ATTAAT 3' TAATTA 5' ---AT TAAT--- 3' 3' ---TAAT TA--- 5' AseI, AsnI, BpoAI, Sru4DI, VspI

PsiI Pseudomonas sp. SE-G49 5' TTATAA 3' AATATT 5' ---TTA TAA--- 3' 3' ---AAT ATT--- 5'

Psp03I Phormidium sp. 5' GGWCC 3' CCWGG 5' ---GGWC C--- 3' 3' ---C CWGG--- 5' Bme216I , CauI , EagMI , FdiI , HgiBI , HgiHIII , SinI , VpaK11AI

Psp5II Pseudomonas sp. RFL5 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5'

Psp6I Pseudomonas sp. B6 5' CCWGG 3' GGWCC 5' --- CCWGG--- 3' 3' ---GGWCC --- 5' AeuI , AglI , ApaORI , Bse17I , EcoRII , Fsp1604I , SspAI, Sth117I

Psp23I Pseudomonas sp. 23 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' ApiI, BspBI, CflI, MhaAI, Pfl21I, PstI, SalPI, Sst12I, YenI

Psp1406I Pseudomonas pseudoalcaligenes RFL1406 5' AACGTT 3' TTGCAA 5' ---AA CGTT--- 3' 3' ---TTGC AA--- 5' Accl

PspAI Pseudomonas sp. 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, CfrJ4I , EaeAI, EclRI, Pac25I, SmaI , TspMI, XcyI, XmaI, XmaCI

PspALI Pseudomonas sp. AL1637 5' CCCGGG 3' GGGCCC 5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI , SmaI , TspMI, XcyI, XmaI, XmaCI

Psp124BI Pseudomonas sp. 124B 5' GAGCTC 3' CTCGAG 5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5'

PspCI Pseudomonas sp. C 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PmlI

PspEI Pseudomonas sp. E 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstPI, Eci125I, Eco91I, EcoO65I, EcoO128I

PspGI 3BM3 Pyrococcus sp. GI-H 5' CCWGG 3' GGWCC 5' --- CCWGG--- 3' 3' ---GGWCC --- 5' AeuI , AjnI, AorI , Bse17I , EcoRII , Fsp1604I , Psp6I, SspAI, Sth117I

PspLI Pseudomonas sp. L 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5'

PspN4I

Pseudomonas sp. N4

5' GGNNCC 3' CCNNGG

5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5'

AspNI, BscBI,	BmiI,	BspLI,
NlaIV

PspOMI Pseudomonas sp. OM2164 5' GGGCCC 3' CCCGGG 5' ---G GGCCC--- 3' 3' ---CCCGG G--- 5' Apal , Bsp120I, Ppel

PspPI Psychrobacter immobilis TA137 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bal228I, BavBII, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I

PspPPI Pseudomonas sp. PP 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5'

PspXI Pseudomonas sp. A1-1 5' VCTCGAGB 3' BGAGCTCV 5' ---VC TCGAGB--- 3' 3' ---BGAGCT CV--- 5'

PsrI Pseudomonas stutzeri N2 5' GAACN ₆ TAC 3' CTTGN ₆ ATG 5' ---GAACN ₆ TACN ₆ NNNNNN --- 3' 3' ---CTTGN ₆ ATGN ₆ N NNNNN--- 5'

PssI Pseudomonas sp. 5' RGGNCCY 3' YCCNGGR 5' ---RGGNC CY--- 3' 3' ---YC CNGGR--- 5'

PstI Providencia stuartii 164 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, BspBI, CfuII, Ecl2zI, HalII, PstI, Sag16I, Sag23I, Sst12I, XcpI

PstNHI

Pseudomonas stutzeri NH

5' GCTAGC 3' CGATCG

5' ---G CTAGC--- 3' 3' ---CGATC G--- 5'

AceII , AsuNHI, BmtI ,	BspOI ,
NheI, LlaG2I

PsuI Pseudomonas stutzeri Mck 28-pH52 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5'

Psu161I Pseudomonas stutzeri 161 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, MvrI, Ple19I, PvuI, RshI, XorII

PsuAI Phormidium subfuscum 5' YACGTR 3' RTGCAY 5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5'

PsyI Pseudomonas syringae Lki 1-pH124 5' GACNNNGTC 3' CTGNNNCAG 5' ---GACN NNGTC--- 3' 3' ---CTGNN NCAG--- 5' AspI, AtsI, PflFI, TelI, Tth111I

Ptal Phormidium tadzschicicum 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, PinBII, Ptal

Pun14627I Phormidium uncinatum 1462/7 5' TGCGCA 3' ACGCGT 5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdII, FspI, MstI, NsbI, PamI

Pun14627II Phormidium uncinatum 1462/7 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'

PunAI Phormidium uncinatum 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BspLU4I, Eco27kI, Nli3877I , PlaAI

PunAII Phormidium uncinatum 5' RCATGY 3' YGTACR 5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5'

PvuI Proteus vulgaris 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa22MI, BspCI, ErhB9I, NblI, Ple19I, Psu161I, RshI, XorII

PvuII 1PVU Proteus vulgaris 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'

Pvu84II Proteus vulgaris 84 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'

R

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer

RalF40I Ruminococcus albus F-40 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI , HacI, MkrAI, Sau3AI, SsiAI

Rcal Rhodococcus capsulatum 5' TCATGA 3' AGTACT 5' ---T CATGA--- 3' 3' ---AGTAC T--- 5'

RflFI Ruminococcus flavefaciens FD-1 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'

RflFII

Ruminococcus flavefaciens FD-1

5' AGTACT 3' TCATGA

5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5'

Acc113I, AssI,	BmcAI, Bpa34I,
DpaI, Eco255I, ScaI, Zrml

RleAI Rhizobium leguminosarum 5' CCCACA 3' GGGTGT 5' ---CCCACAN ₈ NNNN --- 3' 3' ---GGGTGTN ₈ N NNN--- 5'

Rmal Rhodothermus marinus 5' CTAG 3' GATC 5' ---C TAG--- 3' 3' ---GAT C--- 5'

Rme21I Rhizobium meliloti 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BbvAII, BliRI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I

RsaI

Rhodopseudomonas sphaeroides

5' GTAC 3' CATG

5' ---GT AC--- 3' 3' ---CA TG--- 5'

AfaI, Csp6I , CviQI , CviRII ,
HpyBI, PabI , PlaAII,	RsaNI ,

RshI Rhodopseudomonas sphaeroides 2.4.1 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, MvrI, Ple19I, Psu161I, PvuI, XorII

RspLKI Rhodococcus sp. LK2 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5'

RspLKII Rhodococcus sp. LK2 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AliI, AsiI, BamHI , Bce751I, Nsp29132II, OkrAI, RspLKII, SolI

RspXI Rhodococcus sp. 5' TCATGA 3' AGTACT 5' ---T CATGA--- 3' 3' ---AGTAC T--- 5'

RsrI Rhodopseudomonas sphaeroides 5' GAATTC 3' CTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'

RsrII Rhodopseudomonas sphaeroides 5' CGGWCCG 3' GCCWGGC 5' ---CG GWCCG--- 3' 3' ---GCCWG GC--- 5'

Rsr2I Rhodobacter sphaeroides SE-I2 5' CGGWCCG 3' GCCWGGC 5' ---CG GWCCG--- 3' 3' ---GCCWG GC--- 5'

RtrI Rhizobium trifolii 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'

Rtr63I Rhizobium trifolii 63 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'

Anteckningar

Restriktionsmodifieringssystem : restriktionsenzym
Grundläggande koncept	Isoschizomer Neoschizomer Isocaudomer
Igenkänningssekvens 4bp	Taq I Sau3A AluI* Hae III* Dpn II Nla III Hpa II
Igenkänningssekvens 5bp	Eco RII Fok jag
Igenkänningssekvens 6bp	Aaa jag Bgl II PovII* Eco RV* Eco RI Bam HI Hind III Xba I Xho I Sac I Pst I Nde I
Igenkänningssekvens 8bp	Inte jag
Listor	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv CD EF GK LN ELLER S TZ
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