Lista över skärplatser för restriktionsenzym: Bsa–Bso

Legenden om nukleobaser
Koda	Nukleotid representerad
A	Adenin (A)
C	Cytosin (C)
G	Guanin (G)
T	Tymin (T)
N	A, C, G eller T
M	A eller C
R	A eller G
W	A eller T
Y	C eller T
S	C eller G
K	G eller T
H	A, C eller T
B	C, G eller T
V	A, C eller G
D	A, G eller T

Den här artikeln innehåller en lista över de mest studerade restriktionsenzymer vars namn börjar med Bsa till Bso inklusive. Den innehåller cirka 90 enzymer. Följande information ges:

Enzym : Accepterat namn på molekylen , enligt den internationellt antagna nomenklaturen och bibliografiska referenser . (Mer läsning: se avsnittet " Nomenklatur " i artikeln " Restriktionsenzym ".)
PDB-kod : Kod som används för att identifiera strukturen av ett protein i PDB -databasen över proteinstrukturer. 3D-atomstrukturen hos ett protein ger mycket värdefull information för att förstå de intima detaljerna i dess verkningsmekanism.
Källa : Organism som naturligt producerar enzymet.
Igenkänningssekvens : Sekvens av DNA som känns igen av enzymet och till vilken det specifikt binder.
Skärning : Skärställe och DNA-produkter från snittet. Igenkänningssekvensen dussintals nukleotider bort från igenkänningsstället.
Isoschizomerer och neoschizomerer : En isoschizomer är ett enzym som känner igen samma sekvens som ett annat. En neoschizomer är en speciell typ av isoschizomer som känner igen samma sekvens som en annan, men skär på ett annat sätt. Ett maximalt antal av 8-10 vanligaste isoschizomerer är indikerade för varje enzym men det kan finnas många fler. Neoschizomerer visas med fet och grön typsnitt (t.ex.: BamHI ). När "Ingen på datum " anges betyder det att det inte fanns några registrerade isoschizomerer i databaserna det datumet med en tydligt definierad skärplats. Isoschizomerer indikerade med vitt teckensnitt och grå bakgrund motsvarar enzymer som inte finns med i de aktuella listorna:

som i detta ej listade enzym:

EcoR70I

Hela listans navigering

Restriktionsenzymer

Bsa - Bso

Enzym PDB-kod Källa Igenkänningssekvens Skära Isoschizomerer

BsaI Bacillus stearothermophilus 20241 5' GGTCTC 3' CCAGAG 5' ---GGTTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' Bso31I, BspTNI, Eco31I

Bsa29I Bacillus sp. 29 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAII

BsaAI Bacillus stearothermophilus G668 5' YACGTR 3' RTGCAY 5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5'

BsaBI Bacillus stearothermophilus B674 5' GATN ₄ ATC 3' CTAN ₄ TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'

BsaHI Bacillus stearothermophilus CPW11 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamII

BsaJI Bacillus stearothermophilus J695 5' CCNNGG 3' GGNNCC 5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5'

BsaMI Bacillus stearothermophilus M293 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269I

BsaOI Bacillus stearothermophilus O-122 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5'

BsaWI Bacillus stearothermophilus W1718 5' WCCGGW 3' WGGCCW 5' ---W CCGGW--- 3' 3' ---WGGCC W--- 5'

BsaXI

Bacillus stearothermophilus 25B

5' ACN ₅ CTCC 3' TGN ₅ GAGG

5' ---ACN ₅ CTCCN ₆ NNNN --- 3' 3' ---TGN ₅ GAGGN ₆ N NNN--- 5'

BsmCI, BsmDI, BsmXI

Bscl Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, Pgal, SpmI

Bsc4I Bacillus schlegelii 4 5' CCN ₇ GG 3' GGN ₇ CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5'

Bsc91I Bacillus sp. 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'

Bsc107I Bacillus schlegelii 107 5' CCN ₇ GG 3' GGN ₇ CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5'

BscAI Bacillus schlegelii S3 5' GCATC 3' CGTAG 5' ---GCATCNNNN NN--- 3' 3' ---CGTAGNNNNNN --- 5'

BscBI

Bacillus sp. A11

5' GGNNCC 3' CCNNGG

5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5'

AspNI,	BmiI,	BspLI, NlaIV,
PspN4I

BscCI Bacillus sp. 2G 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctI

BscFI Bacillus sp. JY391 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBI

BselI Bacillus stearothermophilus 1 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5'

Bse8I Bacillus sp. 8 5' GATN ₄ ATC 3' CTAN ₄ TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'

Bse15I Bacillus sp. 15 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoI

Bse16I Bacillus sp. 16 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII , MvaI

Bse17I Bacillus sp. 17 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I , PspGI

Bse21I Bacillus sp. 21 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI

Bse24I Bacillus sp. 24 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I , PspGI

Bse64I Bacillus sp. 64 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AspAI, Bse64I, BseT9I, BstPI, Ecal, Eci125I, EcoO65I, NspSAII

Bse118I Bacillus sp. 118 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'

Bse634I 1KNV Bacillus sp. 634 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'

BseAI Bacillus sphaericus 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2I

BseBI Bacillus stearothermophilus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI , AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII , MvaI, SleI , ZanI

BseCI Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaI

BseDI Bacillus stearothermophilus RFL1434 5' CCNNGG 3' GGNNCC 5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5'

Bse3DI Bacillus stearothermophilus 3D 5' GCAATG 3' CGTTAC 5' ---GCAATGNN --- 3' 3' ---CGTTAC NN--- 5'

BseGI Bacillus stearothermophilus Vs 34-031 5' GGATG 3' CCTAC 5' ---GGATGNN --- 3' 3' ---CCTAC NN--- 5'

BseJI Bacillus stearothermophilus Tsp5 5' GATN ₄ ATC 3' CTAN ₄ TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'

BseKI Bacillus stearothermophilus Ra3-212 5' GCAGC 3' CGTCG 5' ---GCAGCN ₇ N NNNN--- 3' 3' ---CGTCGN ₇ NNNNN --- 5' AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I

BseLI Bacillus stearothermophilus LK3-551 5' CCN ₇ GG 3' GGN ₇ CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5'

BseMI Bacillus stearothermophilus Isl 15-111 5' GCAATG 3' CGTTAC 5' ---GCAATGNN --- 3' 3' ---CGTTAC NN--- 5'

BseMII Bacillus stearothermophilus Isl 15-111 5' CTCAG 3' GAGTC 5' ---CTCAGN ₇ NNN --- 3' 3' ---GAGTCN ₇ N NN--- 5'

BseNI Bacillus sp. N 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5'

BsePI Bacillus stearothermophilus P6 5' GCGCGC 3' CGCGCG 5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5'

BseQI Bacillus sp. F 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

BseRI Bacillus sp. R 5' GAGGAG 3' CTCCTC 5' ---GAGGAGN ₇ NNN --- 3' 3' ---CTCCTCN ₇ N NN--- 5'

BseSI Bacillus stearothermophilus Jo-553 5' GKGCMC 3' CMCGKG 5' ---GKGCM C--- 3' 3' ---C MCGKG--- 5'

BseT9I Bacillus sp. T9 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, Bse64I, BstEII, BstT9I, Ecal, Eci125I, EcoO65I, NspSAII, PspEI

BseT10I Bacillus sp. T10 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEI

BseXI Bacillus stearothermophilus Ra3-212 5' GCAGC 3' CGTCG 5' ---GCAGCN ₇ N NNNN--- 3' 3' ---CGTCGN ₇ NNNNN --- 5' AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1I

BseX3I Bacillus stearothermophilus X3 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII

BseYI Bacillus sp. 2521 5' CCCAGC 3' GGGTCG 5' ---C CCAGC--- 3' 3' ---GGGTC G--- 5'

BseZI Bacillus sp. Z 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'

Bsgl Bacillus sphaericus B922 5' GTGCAG 3' CACGTC 5' ---GTGCAGN ₁₂ NNNN --- 3' 3' ---CACGTCN ₁₂ NN NN--- 5'

BshI Bacillus sphaericus 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

Bsh45I

Bacillus sphaericus 45

5' GWGCWC 3' CWCGWG

5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5'

Alw21I, AspHI, Bbv12I, BsiHKAI,

HgiAI,

HpyF46II,

MspV281I

Bsh1236I Bacillus sphaericus RFL1236 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelI

Bsh1285I Bacillus sphaericus RFL1285 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5'

Bsh1365I Bacillus sphaericus RFL1365 5' GATN ₄ ATC 3' CTAN ₄ TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'

BshFI Bacillus sphaericus 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5'

BshGI Bacillus sphaericus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI , SspAI

BshKI Bacillus sphaericus 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12I

BshNI Bacillus sphaericus TK-45 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI

BshTI

Bacillus sphaericus Jo22-024

5' ACCGGT 3' TGGCCA

5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5'

AgeI, AsiaAI,	AsiGI,	CsiAI
CspAI, PinAI

BsiI Bacillus sphaericus 5' CACGAG 3' GTGCTC 5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5'

BsiBI Bacillus sp. 5' GATN ₄ ATC 3' CTAN ₄ TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'

BsiCI Bacillus sp. 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Acpl, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuI

BsiEI Bacillus sp. 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5'

BsiHKAI

Bacillus stearothermophilus

5' GWGCWC 3' CWCGWG

5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5'

Alw21I, AspHI, Bbv12I, Bsh45I,

Bsm6I,

HgiAI, MspV281I

BsiHKCI Bacillus sp. HKC 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I , PlaAI, PunAI

BsiKI Bacillus sp. 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'

BsiLI Bacillus sp. 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII , MvaI

BsiMI Bacillus sp. 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBII

BsiQI

Bacillus sp.

5' TGATCA 3' ACTAGT

5' ---T GATCA--- 3' 3' ---ACTAG T--- 5'

AbaI, BclI, BspXII,
BstT7I, FbaI, Ksp22I,	ParI

BsiSI Bacillus sp. 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5'

BsiWI Bacillus sp. 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5'

BsiXI Bacillus sp. X 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21I

BsiYI Bacillus sp. 5' CCN ₇ GG 3' GGN ₇ CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5'

BsiZI Bacillus sp. 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12I

BslI Bacillus sp. 5' CCN ₇ GG 3' GGN ₇ CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5'

BslFI Bacillus stearothermophilus FI 5' GGGAC 3' CCCTG 5' ---GGGACN ₈ NN NNNN--- 3' 3' ---CCCTGN ₈ NNNNNN --- 5'

BsmI Bacillus stearothermophilus NUB 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctI

BsmAI

Bacillus stearothermophilus A664

5' GTCTC 3' CAGAG

5' ---GTCTCN NNNN--- 3' 3' ---CAGAGNNNNN --- 5'

Alw26I,	BcoDI, BscQII,	BsoMAI,
BstMAI

BsmBI Bacillus stearothermophilus B61 5' CGTCTC 3' GCAGAG 5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5'

BsmFI Bacillus stearothermophilus F 5' GGGAC 3' CCCTG 5' ---GGGACN ₈ NN NNNN--- 3' 3' ---CCCTGN ₈ NNNNNN --- 5'

BsmSI Bacillus stearothermophilus CP114 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'

Bso31I Bacillus stearothermophilus E31 5' GGTCTC 3' CCAGAG 5' ---GGTTCN NNNN--- 3' 3' ---CCAGAGNNNNNN --- 5'

BsoBI 1DC1 Bacillus stearothermophilus JN2091 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I , PlaAI, PunAI

BsoCI Bacillus stearothermophilus AU891 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduI

BsoFI Bacillus stearothermophilus F66 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5'

BsoMAI

Bacillus stearothermophilus MA

5' GTCTC 3' CAGAG

5' ---GTCTCN NNNN--- 3' 3' ---CAGAGNNNNN --- 5'

Alw26I,	BcoDI, BscQII,	BsmAI,
BstMAI

Anteckningar

Restriktionsmodifieringssystem : restriktionsenzym
Grundläggande koncept	Isoschizomer Neoschizomer Isocaudomer
Igenkänningssekvens 4bp	Taq I Sau3A AluI* Hae III* Dpn II Nla III Hpa II
Igenkänningssekvens 5bp	Eco RII Fok jag
Igenkänningssekvens 6bp	Aaa jag Bgl II PovII* Eco RV* Eco RI Bam HI Hind III Xba I Xho I Sac I Pst I Nde I
Igenkänningssekvens 8bp	Inte jag
Listor	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv CD EF GK LN ELLER S TZ
* betyder att klyvningen ger trubbiga ändar